• Latest
  • Trending
  • All
Synthetic energy metabolism enables twin engine for cell

Synthetic energy metabolism enables twin engine for cell

December 4, 2022
Oracle Analytics Cloud Backup: Keep Your Data Safe and Secure

Oracle Analytics Cloud Backup: Keep Your Data Safe and Secure

January 7, 2023
Veeam Backup Cloud Server - The Best for Enterprises

Veeam Backup Cloud Server – The Best for Enterprises

December 31, 2022
Domo marketing analytics

The Domo Marketing Analytics Review: The Most Comprehensive and Powerful Tool Available for Email Marketing

December 30, 2022
What is iCloud Backup? and How to Backup iCloud Fast and Simple

What is iCloud Backup? and How to Backup iCloud Fast and Simple

December 13, 2022
advantages of marketing analytics

The Fantastic Advantages Of Marketing Analytics

December 9, 2022
About Cloud Server and How to Backup Cloud Server 

About Cloud Server and How to Backup Cloud Server 

December 6, 2022
Big Data or Cloud Computing

Big Data or Cloud Computing: Which is Better?

December 6, 2022
Astronomers find cosmic rays driving galaxy’s winds

Astronomers find cosmic rays driving galaxy’s winds

December 5, 2022
Superaerophobic polyethyleneimine hydrogels improve electrochemical hydrogen production by promoting bubble detachment

Superaerophobic polyethyleneimine hydrogels improve electrochemical hydrogen production by promoting bubble detachment

December 4, 2022
Target names chief digital, product officer

Target names chief digital, product officer

December 3, 2022
Kohl’s joins early holiday promos trend with October deals

Kohl’s joins early holiday promos trend with October deals

December 3, 2022
Build-A-Bear redesigns website

Build-A-Bear redesigns website

December 2, 2022
Sunday, February 5, 2023
  • Login
Infodoc.info
  • Home
  • Astronomy & Space
  • Biology
  • Chemistry
  • DTC
  • Marketing
  • Technology
  • Stories
    • Guides
    • How Tos
    • Apple
No Result
View All Result
  • Home
  • Astronomy & Space
  • Biology
  • Chemistry
  • DTC
  • Marketing
  • Technology
  • Stories
    • Guides
    • How Tos
    • Apple
No Result
View All Result
Infodoc.info
No Result
View All Result

Synthetic energy metabolism enables twin engine for cell

by Megusta
December 4, 2022
in Biology
0
Synthetic energy metabolism enables twin engine for cell

Synthetic energy metabolism enables twin engine for cell
Schematic illustration of the two-engine design. Credit: SIAT

A research team led by Prof. Yu Tao from the Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, has proposed a novel synthetic energy system that supports yeast cell growth and the production of highly reduced chemicals.

The research was published in Nature Metabolism on Oct. 27.

Cell metabolism has been optimized for self-proliferation instead of production of specific chemicals in the evolutional process. In contrast, rational design plus metabolic reconfiguration using modern biotechnology enables the transformation of cells into high-yield factories.

For production of highly reduced chemicals, e.g., free fat acids (FFAs) as a kind of biofuel, cells need to consume energy and overcome the stoichiometric constraints of chemical composition between the substrate and target product. Thus, rewiring reductive/energy metabolism becomes an effective solution.

In this study, the synthetic energy system contains three modules: the pentose phosphate (PP) cycle, the trans-hydrogenase cycle and the external respiratory chain. The PP cycle is a repeated decarboxylation cycle, in which large amounts of reductants, e.g., nicotinamide adenine dinucleotide phosphate (NADPH), are generated.

Then the trans-hydrogenase cycle can irreversibly transfer one NADPH into one nicotinamide adenine dinucleotide (NADH) in the cytoplasm. Through the external respiratory chain, energy can be generated from cytosolic NADH.

The researchers tested an evolved pyruvate decarboxylase-deficient strain E1B. After overexpressing the PP cycle and the blocking of glycolysis, the strain could not grow on glucose because of excess NADPH accumulation. Expression of the trans-hydrogenase cycle restored normal cell growth. In the context of the synthetic energy system, cell growth improved instead of being weakened as the endogenous energy system was down-regulated, indicating that the system can become an alternative for supplying energy for better cell growth.

See also  A pheromone that explains why puffer fish spawn on beaches under moonlight 
Powered by Inline Related Posts

As for reductive chemical production, a succinate titer of approximately 3.3 g/L was achieved. Integrated with a ratio fine-tuned between precursors, cofactors and energy, the synthetic energy system raised the yield of FFAs to 40% of the maximum theoretical yield, which is the highest yield reported for Saccharomyces cerevisiae and demonstrates the potential of the system for industrial scale production.

“Energy metabolic reprogramming demonstrates that despite extensive regulation of catabolism in yeast, it is still possible to rewire their energy metabolism,” said Prof. Yu.

Provided by
Chinese Academy of Sciences

Megusta

Megusta

No Result
View All Result

Categories

  • Apple
  • Astronomy & Space
  • Biology
  • Chemistry
  • DTC
  • Guides
  • How Tos
  • Marketing
  • Technology
  • Uncategorized
Infodoc.info

Copyright © 2022 Infodoc.info. All right reserved.

Navigate Site

  • About Us
  • Contact
  • Disclaimers
  • Privacy Policy
  • Terms and Conditons

Follow Us

No Result
View All Result
  • Home
  • Astronomy & Space
  • Biology
  • Chemistry
  • DTC
  • Marketing
  • Technology
  • Stories
    • Guides
    • How Tos
    • Apple

Copyright © 2022 Infodoc.info. All right reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In